prokaryoticexpression相关论文
Cloning of transcription factor gene GMPTF1 for tolerance to phosphorus stavation in soybean (Glycin
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Cloning, Prokaryotic Expression and Monoclonal Antibody Preparation of Sonchus yellow net virus P ge
Sonchus yellow net virus (SYNV) belongs to the Nucleorhabdovirus genus of Rhabdoviri-dae family....
Expression,purification and functional characteristic of a novel fungal immunomodulatory protein fro
Ganoderma atrum fungal immunomodulatory protein(FIP-gat)is a new member of the FIP family.The FIP-gat protein was compos......
Nitrite reductase(NiR)is a critical enzyme involved in nitrogen metabolism in plants.A full-length cDNA sequence of 1,76......
Aflatoxin contamination caused by Aspergillus flavus is one of the major destructive legume diseases of peanut worldwide......
CLONING, CHARACTERIZATION AND EXPRESSION ANALYSIS OF PROTEINS IN DIMOCARPUS LONGANA LOUR.SEEDUNGS UN
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CLONING,PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIEL
NifK gene is a key nitrogenase and plays an important role in the nitrogen fixation process.It is necessary to obtain th......
Ascorbate peroxidase(APX)is a key enzyme for active oxygen scavenging of higher plants,and it plays an important role in......
Characterization and expression patterns of mannose-binding lectin(MMBL)gene in mulberry(Morus multi
Mannose-binding lectin(MBL)is an important natural immune molecule which plays a significant role in plant self-defense ......
Cyprinid herpesvirus II(CyHV-2)is the main pathogen causing herpesviral haematopoietic necrosis disease in Carassius aur......
Cloning and characteristic analysis of a novel aspartic protease gene Asp55 from Trichoderma asperel
Aspartic proteases secreted by fungi belonging to the genus Trichoderma play important roles in biocontrol.It could be i......
The eliciting plant response protein gene Epl1 has been cloned from biocontrol agent Trichoderma asperellum ACCC30536.Th......
Functional analysis of a subtilisin-like serine protease gene from biocontrol fungus Trichoderma har
The subtilisin-like serine protease gene ThSS45 has been cloned from Trichoderma harzianum ACCC30371.Its coding region i......
A novel dirigent protein gene with highly stem-specific expression from sugarcane, response to droug
Dirigent and dirigent-like family proteins contain a number of proteins involved in lignification or in the response to ......
Cloning, expression and purification of 3-Deoxy-D-manno-octulosonate 8-Phosphate Synthase from Phyll
The pectic polysaccharide rhamnogalacturonan Ⅱ (RG-Ⅱ) is present in all plant species.Even though the structures of th......
The ac106 gene from Ophiusa Disjungens nucleopolyhedrovirus was cloned and sequenced.Computer analysis revealed that two......
The bioinformaticsanalyses reveal novel antigen epitopesin Major Outer Membrane Protein (MOMP) of Ch
Purpose: The aim of this study was to predict the T-cell and B-cell epitopes in Major Outer Membrane Protein (MOMP) ofCh......
A MAPKK kinase gene was firstly cloned from Dunaliela salina by RT-PCR and RACE technology.The full length of cDNA for M......
Molecular Characteristics and Ranscriptional Response of Two Metallothionein Genes in Oxya chinensis
Metallothioneins(MTs)are small,cysteine-rich,heavy metal-binding proteins involved in metal homeostasis and detoxificati......
Objective: Plasmodium vivax predominates in South-East Asia and the American continent,causes significant morbidity and ......
Sweet Sorghum is a potential energy plant due to its abilities of fast growth and high biomass.It is beneficial to impro......
Molt-inhibiting hormone from the Chinese mitten crab,Eriocheir sinensis:Cloning,Tissue Expression,an
Molt-inhibiting hormone (MIH), a member of the crustacean hyperglycemic neuropeptide hormone family, inhibits the synthe......
Nuclear prelamin A recognition factor, Narf, was identified by screening a human skeletal muscle library in a yeast twoh......
Objective: To clone and express the Aedes albopictus C-type lectin-2(CTL2)protein.Methods: A putative C-type lectin gene......
Objective Previous studies showed that a yellow protein 1(Aael_YP1)gene was significantly downregulated in Ae.aegypti WB......
Isolation and Identification of a Fungi Producing a Thermostable Xylanase and Prokaryotic Expression
In recent years,xylanase has become a widely used enzyme in feed industry because of its effective degradation of ar......
The Vesicular Stomatitis virus genome contains five single-stranded RNA segments of negative polarity,coding for fiv......
Toll-like receptors(TLRs)are a class of important pattern recognition molecules,which play an important role in natu......
The structural and functional protein VP1 of echovirus is important for echovirus infection.Three antigen epitope-ri......
HPLC and LC-TOF/MS were applied to determine theanine in the roots and leaves in the young and mature Dabieshan wild Cam......
Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the key enzyme of the general phenylpropanoid metabolic pathway.A ......
CLONING, EXPRESSION OF CHLAMYS FARRERI ACUTE VIRAL NECROSIS VIRUS DUTPASE GENE AND THE DETERMINATION
Acute virus necrosis virus (AVNV) was reported as one causative agent responsible for summer mass mortality of adult......
根据GenBank中大肠杆菌的soxS基因序列设计引物,以大肠杆菌ATCC25922的基因组为模板,PCR扩增出约324 bp的soxS基因片段,将所得片段......
根据GenBank中副猪嗜血杆菌（HPS）的P2蛋白基因序列,设计合成1对特异性引物,用P2-PCR方法从HB070214株中克隆了1 077bp的P2蛋白基因。......
根据GenBank已发表粪肠球菌Ace基因（AF260879）的核苷酸序列设计1对引物,通过PCR扩增Ace基因保守序列A片段部分序列,将其克隆到pET-32......
根据GenBank中肉鸡硫氧还蛋白(Trx)基因的cDNA序列及载体pET28a(+)中的多克隆位点设计引物,进行Trx克隆和原核表达载体的构建,并在......
目的 克隆、原核表达C2株蓝氏贾第鞭毛虫(Giardia lamblia,简称贾第虫)的小泛素相关修饰物(small ubiquit in-related modifier,SU......
目的 通过原核表达系统制备裂谷热病毒Gn片段主要抗原区蛋白,获取纯化蛋白,并分析抗原性.方法 将合成的裂谷热Gn基因用PCR的方法扩......
目的 开展SmCaMK Ⅰ(曼氏迭宫绦虫钙/钙调素依赖蛋白激酶Ⅰ)的潜在生物学特征分析和功能研究.方法 利用相关网站和软件对SmCaMK Ⅰ......
目的 在大肠杆菌中克隆表达和纯化寨卡病毒(Zika Virus,ZIKV)包膜糖蛋白(Envelope Protein,E)及第三结构域(Envelope DomainⅢ,ED......
Prokaryotic expression of recombinant human p75NTR-Fc fusion protein and its effect on the neurite o
Objective: To clone, express, and identify the extracellular domain gene of human p75 neurotrophin receptor with IgG-Fc ......
Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclo
To prepare monoclonal antibody specific to epidermal growth factor receptor (EGFR) intracellular domain, its gene was am......
High Level Expression of HLA-A*0203-BSP Fusion Protein in Escherichia coli and Construction of Solub
Major histocompatibility complex (MHC) tetramer technology is critical for characterization of antigen-specific T cells.......
目的:构建、表达、纯化抗血管内皮生长因子(VEGF)的Fab片段(眼明,Vasculizumab),并检测其抗VEGF的活性.方法:首先对轻链(LC)及重链......
通过原核表达系统表达猪传染性胃肠炎病毒( TGEV) S基因不同编码区,以鉴定不同编码区表达产物与TGEV抗体的结合能力。在对TGEV S蛋......
根据鲤疱疹病毒II型(Cyprinid herpesvirus II,CyHV-2)ORF 4基因序列(GenBank：JQ815364．1)设计特异性引物,PCR扩增得到ORF 4基因编码......
Methionine and lysine are restrictive essential amino acids of livestock, they are also the most attentive indexes in th......
目的 构建谷胱甘肽-S-转移酶(GST)和中期因子(MK)融合蛋白的原核表达质粒,并表达和纯化蛋白,制备多克隆抗体.方法 通过RT-PCR技术......
目的 应用分子克隆技术获得纳米级Ag85B蛋白并对其进行鉴定和免疫学特性检测.方法 利用S-layer/Streptag I这一具有自我组装能力的......
目的 从亚洲带绦虫成虫cDNA质粒文库中识别并预测自吞噬相关蛋白(autophagy related protein3-like,APG3)基因,并将该基因进行克隆......
